Designing New Tools for Synthetic Biology in Mammalian Cells
Synthetic Biology aims at developing and applying engineering tools to sense and process endogenous information and to implement robust responses to intracellular conditions. Mammalian synthetic networks build on the conjugation of efforts to increase the number of DNA parts for genetic circuit assembly with minimal counteractive effect with the intracellular context, with the ability to design circuits that are activated when specific endogenous molecules are sensed within the cells. We will show recent results on designing new genetic parts for transcriptional or post-transcriptional genetic circuits design, with particular focus on newly engineered protease-responsive RNA binding proteins as a new potential platform for genetic circuits regulation.